Characterization of Two Temperate Lactobacillus paracasei Bacteriophages: Morphology, Kinetics and Adsorption
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چکیده
Background/Aims: Adsorption and kinetic parameters, latent period, burst size and burst time, are characteristics of phage/host systems and can be affected by several environmental factors. As only few studies have focused on temperate dairy phages, we characterized these parameters on temperate Lactobacillus paracasei phages Φ i Lp 84 and Φ i Lp 1308, infective for probiotic strains. Methods: Phages were characterized by transmission electron microscopy and genomic DNA restriction. Adsorption under different environmental conditions, phage kinetics and efficiency of plating (EOP) were determined using the double-layer titration method. Results: Phages Φ i Lp 84 and Φ i Lp 1308 belong to the Siphoviridae family and have genome sizes of 38 and 34 kbp, respectively. Adsorption was affected by calcium concentration, pH, temperature and host viability, and reached a limit at very high multiplicity of infection. Latency, burst time and burst size were of 85 min, 131 min and 46 for Φ i Lp 84, and 51 min, 92 min and 28 for Φ i Lp 1308, respectively, at 37 ° C. A clear influence of temperature on phage kinetics was observed. Regarding EOP, Φ i Lp 84 produced plaques on only 1 out of 8 strains tested. Conclusion: NoticeReceived: March 20, 2014 Accepted after revision: October 18, 2014 Published online: January 15, 2015 Diego J. Mercanti Santiago del Estero 2829 3000 Santa Fe (Argentina) E-Mail diegojav78 @ gmail.com © 2015 S. Karger AG, Basel 0300–5526/15/0581–0049$39.50/0
منابع مشابه
Characterization of two temperate Lactobacillus paracasei bacteriophages: morphology, kinetics and adsorption.
BACKGROUND/AIMS Adsorption and kinetic parameters, latent period, burst size and burst time, are characteristics of phage/host systems and can be affected by several environmental factors. As only few studies have focused on temperate dairy phages, we characterized these parameters on temperate Lactobacillus paracasei phages Φ iLp84 and Φ iLp1308, infective for probiotic strains. METHODS Phag...
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